BETA-CYCLODEXTRIN DIOL PHASE SILICA MATERIALS FOR DIRECT INJECTION-ANALYSIS OF DRUG ENANTIOMERS IN SERUM BY LIQUID-CHROMATOGRAPHY

Beta-cyclodextrin (CD)/diol phase silica materials have been designed for direct serum injection assays of drug enantiomers. The mixed functional phase (MFP) materials having beta-CD and diol phases were synthesized in four steps: introduction of a 3-chloropropyl phase, introduction of a beta-CD phase by ether linkage, introduction of a 3-glycidoxypropyl phase and hydrolysis of the oxirange ring to a diol phase. The obtained MFP materials were more stable against continuous flow of an eluent including phosphate buffer and organic solvent and/or acid hydrolysis than only beta-CD-bonded materials were. These MFP materials could be used for direct serum injection assays of drug enantiomers. The recovery of drug enantiomers from serum was almost 100%.