IMMOBILIZATION OF ALPHA-AMYLASE INTO PHOTOGRAPHIC GELATIN BY CHEMICAL CROSS-LINKING

被引：12

作者：

BAYRAMOGLU, Z

AKBULUT, U

SUNGUR, S

机构：

[1] MIDDLE E TECH UNIV, DEPT CHEM, ANKARA, TURKEY

[2] UNIV ANKARA, FAC SCI, DEPT CHEM, ANKARA, TURKEY

来源：

BIOMATERIALS | 1992年 / 13卷 / 10期

关键词：

IMMOBILIZATION; ENZYMES; GELATIN; CROSS-LINKING;

D O I：

10.1016/0142-9612(92)90131-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Alpha-amylase was immobilized into photographic gelatin by chemical cross-linking with chromium (III) acetate and chromium (III) sulphate. Cellulose triacetate film strips, enabled simple handling when coated with an alpha-amylase-gelatin mixture, accomplishing a high degree of durability during consecutive immersions into reaction media. The optimum conditions for pH, substrate concentration, temperature, incubation time and storing conditions of free and immobilized alpha-amylase were determined. The effect of use number on activity and the effect of resting on reuse number were also determined. Photographic gelatin was found to be a very efficient natural polymer, due to its extraordinary diffusion characteristics for immobilization as a carrier.

引用

页码：704 / 708

页数：5

共 15 条

[1]

AKBULUT U, 1991, Macromolecular Reports, VA28, P239

[2]

BAYRAMOGLU Z, 1990, THESIS MIDDLE E TU A

[3] POLYESTER FILM STRIPS COATED WITH PHOTOGRAPHIC GELATIN CONTAINING IMMOBILIZED GLUCOSE-OXIDASE HARDENED BY CHROMIUM(III) SULFATE [J].