THE EFFECT OF 20-HYDROXYECDYSONE ON EXPRESSION OF GENES-CODING FOR LOW-MOLECULAR-WEIGHT SILK PROTEINS OF GALLERIA-MELLONELLA

被引:9
作者
GRZELAK, K
KLUDKIEWICZ, B
LASSOTA, Z
机构
[1] Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa
关键词
SILK PROTEINS; 20-HYDROXYECDYSONE; JUVENILE HORMONE; GENE EXPRESSION;
D O I
10.1016/0965-1748(93)90001-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The low molecular weight Galleria silk proteins of 24 and 29-30 kDa are coded by the 1100 nucleotides long mRNAs of the posterior silk gland (PSG) cells. The synthesis of these proteins starts during the first 2 days of the last instar when the endogenous ecdysteroid titre is very low. The synthesis of 24 kDa protein precedes the synthesis of 30 kDa protein. PSGs from day 1 last instar larvae are sensitive to exogenous 20-hydroxyecdysone (20-HE) in vitro at 0.5 mug/ml concentration causing an increase of 1100 nucleotides long mRNAs. The electrophoretic analysis of the in vitro labelling proteins indicates that 20-HE may induce the synthesis of 30 kDa protein. Juvenile hormone II in vitro suppresses the stimulatory effect of 20-HE. PSGs from day 3 last instar larvae are insensitive to exogenous 20-HE in vitro.
引用
收藏
页码:211 / 216
页数:6
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