NEAR-IR SPECTROSCOPIC EXAMINATION OF THIN-LAYER CHROMATOGRAPHY PLATES IN THE DIFFUSE TRANSMITTANCE MODE

被引:9
作者
FONG, A [1 ]
HIEFTJE, GM [1 ]
机构
[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405
关键词
NEAR-IR; DIFFUSE TRANSMISSION; THIN-LAYER CHROMATOGRAPHY; DENSITOMETRY;
D O I
10.1366/0003702944028317
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
The nondestructive detection of analyte spots on high-performance thin-layer chromatography (HPTLC) plates is demonstrated with the use of near-IR spectrometry (NIRS). The resulting hyphenated technique is called Thin-Layer Chromatography-Near-infrared Spectrometry (TLC-NIRS). A transmittance geometry is employed, and a set of 18 interference filters is used for wavelength selectivity. Plate-thickness nonuniformity and varying amounts of water vapor adsorption onto the silica-gel TLC plates were found to be the major complications. Variations in silica-gel thickness could be compensated for by obtaining reference spectral scans of the HPTLC plates before performing the chromatography. The effects of water-vapor adsorption were reduced through use of a partial least-squares calibration model. Detection limits near 1 mug were obtained.
引用
收藏
页码:394 / 399
页数:6
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