PRODUCTION OF RECOMBINANT PROTEINS IN THE FILAMENTOUS FUNGUS TRICHODERMA-REESEI

被引：65

作者：

KERANEN, S

PENTTILA, M

机构：

[1] VTT Biotechnology and Food Research, FIN-02044 VTT Espoo

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1995年 / 6卷 / 05期

关键词：

D O I：

10.1016/0958-1669(95)80088-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential of the filamentous fungus Trichoderma reesei for producing heterologous proteins has recently been demonstrated with a number of secreted proteins. Rate-limiting steps and ways of improving the production have been studied, especially using antibody Fab fragments. Major improvements have been achieved by producing the foreign protein fused to the fungal cellulase cellobiohydrolase I. In addition to the strong inducible cbh I promoter, other promoters, which are regulated in a different manner, have been developed.

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页码：534 / 537

页数：4

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