INHIBITION OF BETA-LACTAMASES BY TAZOBACTAM AND INVITRO ANTIBACTERIAL ACTIVITY OF TAZOBACTAM COMBINED WITH PIPERACILLIN

The in-vitro synergistic activity of tazobactaxn, a new β-lactamase inhibitor, combined with piperacillin was tested against various β-lactamase-producing strains. The β-lactamase inhibitory activity of tazobactam against various known types of β-lactamase was also tested in comparison with clavulanic acid or sulbactam. Tazobactam caused a remarkable reduction of the piperacillin MICs for penicillinase and oxyiminocephalosporinase-producing strains and also showed a moderate synergistic effect against cephalosporinase-producing strains. The bactericidal activity of piperacillin was enhanced in combination with tazobactam. Tazobactam inhibited the penicillinases, and the oxyiminocephalosporinase produced by Proteus vulgaris, at low concentration. In these cases its activity was comparable with that of clavulanic acid and stronger than that of sulbactam. Tazobactam demonstrated a better inhibitory capability than sulbactam against the cephalosporinases tested. Tazobactam was able to inactivate intracellular β-lactamase in Prot. vulgaris and Morganella morganii, confirming its ability to penetrate the cell membrane of these species. © 1990 The British Society for Antimicrobial Chemotherapy.