PICOSECOND SINGLE PHOTON TIMING MEASUREMENTS WITH A PROXIMITY TYPE MICROCHANNEL PLATE PHOTOMULTIPLIER AND GLOBAL ANALYSIS WITH REFERENCE CONVOLUTION

被引:59
作者
BOENS, N [1 ]
TAMAI, N [1 ]
YAMAZAKI, I [1 ]
YAMAZAKI, T [1 ]
机构
[1] HOKKAIDO UNIV,FAC ENGN,DEPT CHEM PROC ENGN,SAPPORO,HOKKAIDO 060,JAPAN
关键词
D O I
10.1111/j.1751-1097.1990.tb08702.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abstract— –The use of a proximity type 12 µm microchannel plate photomultiplier (Hamamatsu R1564U) is demonstrated for the accurate determination of picosecond lifetimes of xanthione, stiff stilbene, cryptocyanine, pinacyanol chloride, erythrosin and l, l′‐diethyl‐2,2′‐dicarbocyanine iodide in solution at room temperature. Single curve and global analyses with reference convolution give statistically acceptable mono‐exponential fits to all obtained decays. The count rate and wavelength dependence of the measured excitation pulse profile are discussed. Matching the count rates of sample and reference is recommended for reliable picosecond lifetime determinations. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码:911 / 917
页数:7
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