INVIVO FATE OF MMS-INDUCED DNA LESIONS THAT ELICIT SCE

被引：7

作者：

MORALESRAMIREZ, P

RODRIGUEZREYES, R

VALLARINOKELLY, T

机构：

[1] Departamento de Radiobiología, Instituto Nacional de Investigaciones Nucleares, Mexico, D.F.

来源：

MUTATION RESEARCH | 1992年 / 272卷 / 03期

关键词：

SISTER-CHROMATID EXCHANGE; METHYL METHANESULFONATE; 3-WAY DIFFERENTIAL STAINING;

D O I：

10.1016/0165-1161(92)91534-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A previously reported in vivo protocol, which uses three-way differential staining (TWD) of sister chromatids, allows the screening of mutagen-induced sister-chromatid exchange (SCE) in each of the two cell divisions after mutagen treatment and also those occurring at apparently the same locus in both divisions. In the present work the effect of methyl methanesulfonate (MMS) was studied by means of this protocol. The results showed that MMS-induced DNA lesions that cause SCE are persistent. Some lesions were induced in the second division, as was inferred from the analysis of the response in single cells. The data also indicate that bromodeoxyuridine reduces DNA sensitivity to SCE induction by MMS.

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收藏

页码：215 / 221

页数：7

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