APPLICABILITY OF THE PCR TECHNIQUE IN THE FOOD TESTING LABORATORY - IDENTIFICATION OF LISTERIA-MONOCYTOGENES

被引：4

作者：

BLAIS, BW

PHILLIPPE, LM

BURZYNSKI, M

BIELECKI, J

机构：

[1] Laboratory Services Division, Food Production and Inspection Branch, Agriculture and Agri-Food Canada, Ottawa, K1A 0C6, Ontario

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 09期

关键词：

D O I：

10.1007/BF00156346

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymerase chain reaction (PCR) targeting Listeria monocytogenes-specific hlyA gene sequences was evaluated as a tool for the identification of presumptive positive colonies isolated on Palcam or Oxford agar during routine analysis of food samples by culture technique. The PCR correctly identified L monocytogenes isolates from a large number of samples, and provided a significant saving in the time, labour and cost compared to the traditional biochemical tests.

引用

页码：629 / 632

页数：4

共 6 条

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