OBJECTIVE IDENTIFICATION OF HYPERACTIVATED HUMAN SPERMATOZOA BY COMPUTERIZED SPERM MOTION ANALYSIS WITH THE HAMILTON-THORN SPERM MOTILITY ANALYZER

被引：33

作者：

GRUNERT, JH ^{[1
]}

DEGEYTER, C ^{[1
]}

NIESCHLAG, E ^{[1
]}

机构：

[1] UNIV MUNSTER,INST REPROD MED,STEINFURTER STR 107,W-4400 MUNSTER,GERMANY

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 05期

关键词：

Hamilton-Thorn analyser; Human spermatozoa; Hyperactivity;

D O I：

10.1093/oxfordjournals.humrep.a137151

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Sperm from 10 fertile donors were incubated for 8 h in a capacitating medium (BWW 3.5% HSA) and treated with 1.25μ of 2 mM Ca-ionophore solution. Sperm motion was analysed using the Hamilton - Thorn system before and after incubation and treatment. The acrosome reaction was detected with PSA-FITC labelling of the acrosome. Critical values of sperm movement parameters were defined for objective identification of hyperactive movement by the Hamilton - Thorn system. The incidence of hyperactive movement in the sperm suspensions was '2%. No significant changes of hyperactive movement could be observed after the incubation period or the ionophore treatment, in contrast to a significant rise in the percentage of acrosome-reacted cells after ionophore treatment. The implications of these findings for monitoring hyperactive cells to test sperm function are discussed. © 1990 Oxford University Press.

引用

页码：593 / 599

页数：7

共 32 条

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