USE OF ARBITRARY PRIMER PCR TO TYPE CLOSTRIDIUM-DIFFICILE AND COMPARISON OF RESULTS WITH THOSE BY IMMUNOBLOT TYPING

被引:45
作者
KILLGORE, GE [1 ]
KATO, H [1 ]
机构
[1] GIFU UNIV,SCH MED,INST ANAEROB BACTERIOL,GIFU 500,JAPAN
关键词
D O I
10.1128/JCM.32.6.1591-1593.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An arbitrarily primed PCR (AP-PCR) assay was used to type Clostridium difficile isolates from a hospital outbreak of antibiotic-associated diarrhea. Forty-one isolates were separated into nine groups, with 66% falling into one group; no other group contained more than 10%. Comparison of AP-PCR grouping with that when the immunoblot technique was used showed agreement for 33 of 34 isolates typed by both techniques, and AP-PCR grouped seven isolates that were not typeable by immunoblotting.
引用
收藏
页码:1591 / 1593
页数:3
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