NUCLEAR-DNA CONTENT OF THE SOLANUM SPP IN THE SERIES ETUBEROSA AS DETERMINED BY LASER FLOW-CYTOMETRY

Nuclear DNA content was determined in three accessions of Solanum brevidens, three accessions of S. etuberosum, and one accession of S. fernandezianum, which are diploid (2n = 2x = 24), closely related, non tuber-bearing wild potato species belonging to the series Etuberosa (Solanaceae). The plants were grown in vitro at 18 degrees C or at 25 degrees/22 degrees C (day/night). S. brevidens was also grown in soil in the glasshouse at 25 degrees/19 degrees C (day/night), and in growth chambers at 18 degrees C or 32 degrees C. Leaf nuclei were isolated using a chopping method and stained with propidium iodide. Chicken red blood cells (CRBC; 2.33 pg) were added to the samples of nuclei as internal standards. The fluorescence of plant nuclei relative to CRBC was measured with an EPICS PROFILE flow cytometer. The 2C values of in vitro-grown S. brevidens and S. etuberosum were similar (1.48-1.54 pg, depending on the accession), but they were smaller than the 2C value of S. fernandezianum (1.63 pg). The 2C values of S. brevidens and S. etuberosum were generally smaller than those of the diploid species S. berthaultii (1.60-1.61 pg) and the diploid clones of S. tuberosum (1.60-1.72 pg). A similar relative difference of nuclear DNA content was found also between tetraploid S. brevidens and tetraploid S. tuberosum (2C = 3.15-3.16 pg and 3.50-3.62 pg, respectively). High (32 degrees C) and low (18 degrees C) growth temperatures caused abnormal changes in morphology and reduced fertility in S. brevidens in the growth chamber. The 2C values of S. brevidens grown at 25 degrees/19 degrees C (day/night) or at 32 degrees C were similar, whereas the 2C values were c. 10% lower at 18 degrees C.