KRINGLE-1 DOMAIN OF HUMAN TISSUE-TYPE PLASMINOGEN-ACTIVATOR IS A FUNCTIONAL MODULE MEDIATING FIBRINOGEN-STIMULATED PLASMINOGENOLYTIC ACTIVITY

被引：4

作者：

STERN, A ^{[1
]}

WEIDLE, UH ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM GMBH,DEPT GENET,NONNENWALD 2,POSTFACH 1152,W-8122 PENZBERG,GERMANY

来源：

GENE | 1990年 / 87卷 / 02期

关键词：

Chinese hamster ovary cells; DNA mediated gene transfer; exon; fibrin stimulation; functional domains; intron; oligodeoxynucleotide-mediated mutagenesis; Western blotting;

D O I：

10.1016/0378-1119(90)90318-L

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The functions of the finger and kringle-2 (K2) domains of human tissue-type plasminogen activator (t-PA) in mediating fibrin-stimulated plasminogenolytic activity are well documented. Contradictory results have been reported for the kringle-1 (K1) domain with respect to this property. To clarify this issue we have deleted the finger and the K2 domains of t-PA according to the exon-intron organization of the gene by site-directed mutagenesis. The resulting derivative (GK1L) was constitutively expressed in permanent clones of Chinese hamster ovary cells. The secreted proteins have been partially purified and characterized by Western blotting. Since the plasminogenolytic activity of GK1L is stimulated by fibrin, the K1 domain of t-PA must be a functional domain in this context. © 1990.

引用

页码：305 / 308

页数：4

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