MONOCLONAL-ANTIBODIES TO PREGNANEDIOL-3-GLUCURONIDE - APPLICATION TO A DIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF URINE

被引：20

作者：

LEWIS, JG

CLIFFORD, JK

ELDER, PA

机构：

[1] Steroid Unit, Department of Clinical Biochemistry, Christchurch Hospital, Christchurch

来源：

STEROIDS | 1990年 / 55卷 / 07期

关键词：

ELISA; monoclonal antibodies; pregnanediol-3-glucuronide; steroids;

D O I：

10.1016/0039-128X(90)90035-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal antibodies to pregnanediol-3-glucuronide were produced and characterized. One of three clones investigated provided antibody suitable for a direct urinary enzyme-linked immunosorbent assay (ELISA). The ELISA uses a pregnanediol-thyroglobulin conjugate adsorbed onto the wells of a standard 96-well microtiter plate. Pregnanediol-3-glucuronide in standards or diluted urine competes with the immobilized steroid for antibody-binding sites. After washing, mouse monoclonal antibody bound to the plate is probed with antimouse immunoglobulin peroxidase. After further washing, o-phenylenediamine substrate is added and, finally, the absorbance is read at 492 nm. The ELISA shows excellent performance and agreement with the previous gas Chromatographic method. The ELISA is ideal for aiding the assessment of ovarian function in the routine laboratory. © 1990.

引用

页码：314 / 318

页数：5

共 18 条

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