AMMONIUM ION AT LOW CONCENTRATION STABILIZES THE G-QUADRUPLEX FORMATION BY TELOMERIC SEQUENCE

被引：46

作者：

NAGESH, N ^{[1
]}

CHATTERJI, D ^{[1
]}

机构：

[1] CTR CELLULAR & MOLEC BIOL,HYDERABAD 500007,ANDHRA PRADESH,INDIA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1995年 / 30卷 / 01期

关键词：

G-TETRAD; NH4(I); STABILITY; THERMAL TREATMENT; CD; FLUORESCENCE;

D O I：

10.1016/0165-022X(94)00057-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A synthetic analog of telomeric DNA, d(T(6)G(16)) or d(G(4)T(2)G(4)T(2)G(4)T(2)G(4)) was found to form G-quadruplex structure in NH4-ion environments as low as 1 mM. Other counter ions like K(I) or Sr(II) known to have a stabilizing effect on G-tetrad require much higher concentrations. The multiconformational form of G-tetrad could be identified in NH4(I) upon heating the sample up to 100 degrees C. Circular dichroism spectral studies indicated that on thermally treating the complex NH4(I) ion helps in intramolecular G-tetrad formation over intermolecular association, However the G-tetrad structure formed in NH4(I) was found to be unstable in the presence of intercalator Actinomycin D or Tb(III) ion. It appears that the ionic radius of NP4(I) influences tight fitting of this ion inside the cavity of the tetrad. The stability of G-tetrad in the presence of very low concentration of NH4(I) may have potential applications in future.

引用

页码：1 / 8

页数：8

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