LIPID-PEROXIDATION AND ACTIVITY OF ANTIOXIDANT ENZYMES IN DIABETIC RATS

被引：319

作者：

KAKKAR, R

KALRA, J

MANTHA, SV

PRASAD, K

机构：

[1] ROYAL UNIV HOSP, DEPT PATHOL, SASKATOON, SK S7N 0W8, CANADA

[2] UNIV SASKATCHEWAN, COLL MED, DEPT PATHOL, SASKATOON, SK S7N 0W8, CANADA

[3] UNIV SASKATCHEWAN, COLL MED, DEPT PHYSIOL, SASKATOON, SK S7N 0W8, CANADA

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1995年 / 151卷 / 02期

关键词：

DIABETES; LIPID-PEROXIDATION; THIOBARBITURIC ACID REACTIVE SUBSTANCES; CATALASE; GLUTATHIONE PEROXIDASE; SUPEROXIDE DISMUTASE; OXIDATIVE STRESS;

D O I：

10.1007/BF01322333

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We hypothesized that oxygen free radicals (OFRs) may be involved in pathogenesis of diabetic complications. We therefore investigated the levels of lipid peroxidation by measuring thiobarbituric acid reactive substances (TEARS) and activity of antioxidant enzymes [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] in tissues and blood of streptozotocin (STZ)-induced diabetic rats. The animals were divided into two groups: control and diabetic. After 10 weeks (wks) of diabetes the animals were sacrificed and liver, heart, pancreas, kidney and blood were collected for measurement of various biochemical parameters. Diabetes was associated with a significant increase in TEARS in pancreas, heart and blood. The activity of CAT increased in liver, heart and blood but decreased in kidney. GSH-Px activity increased in pancreas and kidney while SOD activity increased in liver, heart and pancreas. Our findings suggest that oxidative stress occurs in diabetic state and that oxidative damage to tissues may be a contributory factor in complications associated with diabetes.

引用

页码：113 / 119

页数：7

共 43 条

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