BILE-ACID N-ACETYLGLUCOSAMINIDES - FORMATION BY MICROSOMAL N-ACETYLGLUCOSAMINYLTRANSFERASES IN HUMAN LIVER AND KIDNEY

被引：17

作者：

MATERN, H

BOLZ, R

MARSCHALL, HU

SJOVALL, J

MATERN, S

机构：

[1] RHEIN WESTFAL TH AACHEN,DEPT INTERNAL MED 3,AACHEN,GERMANY

[2] KAROLINSKA INST,DEPT PHYSIOL CHEM,S-10401 STOCKHOLM 60,SWEDEN

来源：

FEBS LETTERS | 1990年 / 270卷 / 1-2期

关键词：

Bile acid; Glycosidic conjugation; Glycosyltransferase;

D O I：

10.1016/0014-5793(90)81223-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bile acid N-acetylglucosaminyltransferase activity has been identified in microsomes from human liver and kidney. In both organs the transferases required UDP-N-aeetylglucosamine as sugar donor and were mainly active towards ursodeoxycholic acid. Minor activities were observed towards amidated ursodeoxycholic, hyodeoxycholic and β-muricholic acids. No N-acetylglucosaminidation was detectable with the major primary and secondary bile acids suggesting a specific requirement of the enzymes for bile acids containing 7β- or 6α-hydroxyl groups. Kinetic parameters and other catalytic properties of liver and kidney microsomal N-acetylglucosaminyltransferase activities towards ursodeoxycholic acid are described. © 1990.

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页码：11 / 14

页数：4

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