ETHANAL ASSAY, USING AN ENZYMO-CONDUCTIMETRIC METHOD

被引：10

作者：

SAAD, I

WALLACH, JM

机构：

[1] Laboratoire de Biochimie Analytique, I.C.B.M.C., Bâtiment 303

来源：

ANALYTICAL LETTERS | 1992年 / 25卷 / 01期

关键词：

ETHANOL; ACETALDEHYDE; ALDEHYDE DEHYDROGENASE; CONDUCTIMETRY;

D O I：

10.1080/00032719208020006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzymo-conductimetric method for the assay of ethanal is described. It is based on ethanal oxidation in presence of yeast aldehyde dehydrogenase. Experimental conditions compatible with conductimetry were optimized and kinetic parameters of the enzymatic reaction determined. Due to the low K(M) value of ethanol for the enzyme, an end-point assay was proposed. A linear relationship was demonstrated between experimental conductance changes and ethanol concentration up to 25-mu-M. Validation of the assay was made on wines by comparison with a spectrophotometric method. Both methods gave similar results, but conductimetry avoids sample treatment if solutions are coloured or turbid.

引用

页码：37 / 48

页数：12

共 20 条

[1] CONDUCTIMETRIC MEASUREMENTS OF ENZYME-ACTIVITIES [J].