RELATIVE ORDER DETERMINATION OF 4 YP COSMIDS ON METAPHASE AND INTERPHASE CHROMOSOMES BY 2-COLOR COMPETITIVE INSITU HYBRIDIZATION

被引：23

作者：

SLIM, R ^{[1
]}

WEISSENBACH, J ^{[1
]}

NGUYEN, VC ^{[1
]}

DANGLOT, G ^{[1
]}

BERNHEIM, A ^{[1
]}

机构：

[1] INST PASTEUR,UNITE GENET MOLEC HUMAINE,F-75014 PARIS,FRANCE

来源：

HUMAN GENETICS | 1991年 / 88卷 / 01期

关键词：

D O I：

10.1007/BF00204923

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two-color competitive in situ hybridization was used to cytogenetically order four Yp cosmid probes, located in the pseudo-autosomal and TDF regions. The probes were hybridized by pairs to metaphase and interphase chromosomes. On metaphase chromosomes, determination of order between sequences separated by 3 Mb from each other was possible on a statistical basis, whereas the relative position of sequences 0.6 Mb apart could not be determined. On interphase chromosomes the complete order between sequences separated by 0.6-6 Mb was obtained rapidly by measuring the distances between two cosmid spots of every cosmid pair used in 28 to 60 nuclei. Results demonstrate the potential power of fluorescent in situ hybridization at interphase for high resolution cosmid mapping.

引用

页码：21 / 26

页数：6

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