HIV protease is a homodimeric protein whose activity is essential to viral function. We have investigated the molecular dynamics of the HIV protease, thought to be important for proteinase function, bound to high affinity inhibitors using NMR techniques. Analysis of N-15 spin refaxation parameters, of all but 13 backbone amide sites, reveals the presence of significant internal motions of the protein backbone. In particular, the flaps that cover the proteins active site of the protein have terminal loops that undergo large aCnpIitude motions on the ps to ns time scale, while the tips of the flaps undergo a conformational exchange on the mu s time scale. This enforces the idea that the flaps of the proteinase are flexible structures that facilitate function by permitting substrate access to and product release from the active site of the enzyme.
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
CLORE, GM
SZABO, A
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机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
SZABO, A
BAX, A
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机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
BAX, A
KAY, LE
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机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
KAY, LE
DRISCOLL, PC
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机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
DRISCOLL, PC
GRONENBORN, AM
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机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
CLORE, GM
SZABO, A
论文数: 0引用数: 0
h-index: 0
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
SZABO, A
BAX, A
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h-index: 0
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
BAX, A
KAY, LE
论文数: 0引用数: 0
h-index: 0
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
KAY, LE
DRISCOLL, PC
论文数: 0引用数: 0
h-index: 0
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda
DRISCOLL, PC
GRONENBORN, AM
论文数: 0引用数: 0
h-index: 0
机构:Laboratory of Chemical Physics, Building 2 National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health, Maryland, 20892, Bethesda