AN IMPROVED SILVER STAINING PROCEDURE FOR SCHIZODEME ANALYSIS IN POLYACRYLAMIDE GRADIENT GELS

被引：21

作者：

GONCALVES, AM

NEHME, NS

MOREL, CM

机构：

[1] Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Brasil, Rio de Janeiro

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1990年 / 85卷 / 01期

关键词：

D O I：

10.1590/S0074-02761990000100017

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels are applied to gradient gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.

引用

页码：101 / 106

页数：6

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Goncalves A. M., 1984, Genes and antigens of parasites. A laboratory manual. Proceedings of a course held 14 November-17 December 1983, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil., P95

[4] DETECTION OF SUBNANOGRAM QUANTITIES OF DNA AND RNA ON NATIVE AND DENATURING POLYACRYLAMIDE AND AGAROSE GELS BY SILVER STAINING [J].