ENDOMETRIAL ASSESSMENT IN A GROUP OF INFERTILE WOMEN ON STIMULATED CYCLES FOR IVF - IMMUNOHISTOCHEMICAL FINDINGS

被引：27

作者：

MANNERS, CV

机构：

[1] Bourn Hall Clinic, Bourn

[2] Physiological Laboratory, Cambridge CB2 3EG, Downing Street

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 02期

基金：

英国医学研究理事会;

关键词：

24K protein; Endometrial biopsy; Histology; Immuno-histochemistry; α2-PEG;

D O I：

10.1093/oxfordjournals.humrep.a137055

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

An immunohistochemical assessment of the endometrium was carried out in a group of IVF patients on stimulated cycles, in order to evaluate this technique against standard histo-logical methods and to consider its application in a clinical situation. Monoclonal antibodies to the two cycle-dependent proteins: pregnancy associated endometrial α2-globulin (α2-PEG) and 24K (a protein originally isolated from an oestrogen-dependent breast tumour line, MCF-7) were used in the experiment. Immunohistochemical results concerning the effect of drug stimulation, age and date of biopsy on the secretory state of the endometrium revealed trends which were consistent with previous histological data, helping to confirm the value of this new technique. In addition, several specimens were found to have a normal, i.e. in phase, histological appearance but to have an atypical pattern of protein secretion. These observations suggest that biochemical monitoring of the uterus should be used in conjunction with routine histological dating. © 1990 Oxford University Press.

引用

页码：128 / 132

页数：5

共 19 条

[1]

Bell S.C., Secretory endometrial/decidual proteins and their function in early pregnancy, J. Reprod. Fertil, 36, pp. 109-125, (1988)

[2]

Birkenfeld A., Beier H.M., Schenker J.G., Review: The effect of clomiphene citrate on early embryonic development, endometrium and implantation, Hum. Reprod., 1, pp. 387-395, (1986)

[3]

Ciocca D.R., Adams D.J., Edwards D.P., Bjercke R.J., McGuire W.L., Distribution of an estrogen-induced protein with a molecular weight of 24, 000 in normal and malignant human tissues and cells, Cancer Res, 43, pp. 1204-1210, (1983)

[4]

Ciocca D.R., Asch R., Adams D.J., McGuire W.L., Evidence for modulation of a 24K protein in human endometrium during the menstrual cycle, J. Clin. Endocrinol. Metab, 57, pp. 496-499, (1983)

[5]

Cordell J.L., Falini B., Erber W., Ghosh A.K., Abdulaziz Z., Macdonald S., Pulford K., Stein H., Mason D.Y., Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes), J. Histochem. Cytochem, 32, pp. 219-229, (1984)

[6]

Dallenbach-Hellweg G., The endometrium in natural and artificial luteal phases. Hum, Reprod, 3, pp. 165-168, (1988)

[7]

Davies M.C., Erson M.C., Mason B.A., Jacobs H.S., Successful fertility treatment by ovum donation: The ultimate test of uterine competence, N. Engl, J. Med, (1989)

[8]

Edwards D.P., Adams D.J., Savage N., McGuire W.L., Estrogen induced synthesis of specific proteins in human breast cancer cells, Biochem. Biophys. Res. Commun, 93, (1980)

[9]

Graf M.J., Reyniak J.V., Battle-Mutter P., Laufer N., Histologic evaluation of the luteal phase in women following follicle aspiration for oocyte retrieval, Fertil. Steril, 49, pp. 616-619, (1988)

[10]

Julkunen M., Seppala M., Janne O.A., Complete amino acid sequence of human placental protein 14: A progesterone-regulated uterine protein homologus to /3-lactoglobulins, Proc. Natl. Acad. Sci. USA, 85, (1988)

← 1 2 →