IDENTIFICATION AND LOCALIZATION OF CARBONIC-ANHYDRASE IN 2 CHLORELLA SPECIES

被引：13

作者：

COLEMAN, JR ^{[1
]}

ROTATORE, C ^{[1
]}

WILLIAMS, TG ^{[1
]}

COLMAN, B ^{[1
]}

机构：

[1] YORK UNIV,DEPT BIOL,N YORK M3J 1P3,ONTARIO,CANADA

来源：

PLANT PHYSIOLOGY | 1991年 / 95卷 / 01期

关键词：

D O I：

10.1104/pp.95.1.331

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Carbonic anhydrase (CA) activity and localization have been examined in two species of the eukaryotic green alga Chlorella. Mass spectrometric and potentiometric assays of CA activity indicate that C. ellipsoidea contains very little extracellular CA activity whereas C. saccharophila exhibits significant extracellular activity appears to be correlated with the presence of a 36 kilodalton polypeptide that was detected immunologically using a polyclonal antibody directed against the 37 kilodalton Chlamydomonas CA monomer. Both Chlorella species and enzymatically isolated C. ellipsoidea chloroplasts also contain an immunologically similar 38 kilodalton polypeptide that may be a cytosolic or chloroplastic form of CA.

引用

页码：331 / 334

页数：4

共 11 条

[1] EFFECT OF CO2 CONCENTRATION ON PROTEIN-BIOSYNTHESIS AND CARBONIC-ANHYDRASE EXPRESSION IN CHLAMYDOMONAS-REINHARDTII [J].