INDUCTION OF HUMAN AUTOLOGOUS CYTOTOXIC T-LYMPHOCYTES ON FORMALIN-FIXED AND PARAFFIN-EMBEDDED TUMOR SECTIONS

被引：91

作者：

LIU, SQ

SAIJO, K

TODOROKI, T

OHNO, T

机构：

[1] UNIV TSUKUBA,SCH MED,INST PHYS & CHEM RES,RIKEN,CELL BANK,TSUKUBA,IBARAKI 305,JAPAN

[2] UNIV TSUKUBA,SCH MED,DEPT SURG,TSUKUBA,IBARAKI 305,JAPAN

来源：

NATURE MEDICINE | 1995年 / 1卷 / 03期

关键词：

D O I：

10.1038/nm0395-267

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human autologous tumour-specific cytotoxic T lymphocytes (CTL) were generated from peripheral blood on small formalin-fixed paraffin-embedded sections of a gastric cancer. The CTL killed live target cells at an effector/target ratio of 1 within 24 hours and showed the same target specificity as those induced on live cancer cells, The killing activity of the CTL lasted for more than four months in culture and was inhibited by antibodies against CD8 and MHC-class I. These results suggest that adoptive immunotherapy of tumours will be possible with CTL induced on a stable source of tumour antigen.

引用

页码：267 / 271

页数：5

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