OLIGODEOXYNUCLEOSIDE PHOSPHOROTHIOATE STABILITY IN SUBCELLULAR EXTRACTS, CULTURE MEDIA, SERA AND CEREBROSPINAL-FLUID

被引：204

作者：

CAMPBELL, JM

BACON, TA

WICKSTROM, E

机构：

[1] UNIV S FLORIDA,DEPT CHEM,TAMPA,FL 33620

[2] UNIV S FLORIDA,DEPT BIOCHEM & MOLEC BIOL,TAMPA,FL 33620

[3] UNIV S FLORIDA,DEPT SURG,TAMPA,FL 33620

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 20卷 / 03期

关键词：

Deoxyribonuclease; DNA derivative; Fetal bovine serum; HeLa cell lysate; Human serum; Nuclease resistance; Rabbit reticulocyte lysate; Rat cerebrospinal fluid;

D O I：

10.1016/0165-022X(90)90084-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Degradation of a synthetic oligodeoxynucleoside phosphorothioate was studied in six systems used for antisense inhibition experiments. Oligodeoxynucleoside phosphorothioates were degraded very slowly at 37°C in all of the systems studied. Measured half-lives of pentadecamers were 12±1 h in rabbit reticulocyte lysate, 7±1 h in HeLa cell postmitochondrial extract, 14±2 h in RPMI 1640 with 10% fetal bovine serum, 8±1 h in undiluted fetal bovine serum, 9±1 h in adult human serum, and 19±7 h in rat cerebrospinal fluid. © 1990.

引用

页码：259 / 267

页数：9

共 12 条

[1]

ALPHA-OLIGODEOXYNUCLEOTIDE STABILITY IN SERUM, SUBCELLULAR EXTRACTS AND CULTURE MEDIA [J].