PREPARATION AND PURIFICATION OF CHLOROPHYLLS, BACTERIOCHLOROPHYLLS AND OF THEIR DERIVATIVES

被引：8

作者：

BERGER, G ^{[1
]}

KLEO, J ^{[1
]}

ANDRIANAMBININTSOA, S ^{[1
]}

NEUMANN, JM ^{[1
]}

LEONHARD, M ^{[1
]}

机构：

[1] UNIV FREIBURG,INST BIOPHYS & STRAHLENBIOL,W-7800 FREIBURG,GERMANY

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 02期

关键词：

D O I：

10.1080/01483919008049547

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid purification of chlorophylls and bacteriochlorophylls (2–4 hours), at the level of 20100 mg. is described, combining preseparation on reversed phase cartridges and purification on C18 preparative HPLC columns. Several derivatives (pyro, oxidized or reduced compounds. pheophytins) are prepared from these chlorophylls and purified by HPLC. U.V. visible, infrared and NMR spectroscopy are used for identification of these derivatives and analytical HPLC for the evaluation of their purity. Copyright © 1990 by Marcel Dekker, Inc.

引用

页码：333 / 344

页数：12

共 11 条

[1] A RAPID PREPARATIVE METHOD FOR PURIFICATION OF BACTERIOCHLOROPHYLL-A AND BACTERIOCHLOROPHYLL-B [J].

BERGER, G ;

WOLLENWEBER, AM ;

KLEO, J ;

ANDRIANAMBININTSOA, S ;

MANTELE, WG .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1987, 10 (07) :1519-1531

[2]

CAPLE MB, 1978, J BIOL CHEM, V253, P6730

[3] POLYETHYLENE POWDER AS A STATIONARY PHASE FOR PREPARATIVE-SCALE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

CHOW, HC ;

CAPLE, MB ;

STROUSE, CE .

JOURNAL OF CHROMATOGRAPHY, 1978, 151 (03) :357-362

[4] RELATIVE ENRICHMENT OF P-870 IN PHOTOSYNTHETIC REACTION CENTERS TREATED WITH SODIUM-BOROHYDRIDE [J].

DITSON, SL ;

DAVIS, RC ;

PEARLSTEIN, RM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 766 (03) :623-629

[5] FOURIER-TRANSFORM INFRARED SPECTROELECTROCHEMISTRY OF THE BACTERIOCHLOROPHYLL-A ANION RADICAL [J].

MANTELE, W ;

WOLLENWEBER, A ;

RASHWAN, F ;

HEINZE, J ;

NABEDRYK, E ;

BERGER, G ;

BRETON, J .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1988, 47 (03) :451-455

[6] PREPARATION + PROPERTIES OF PYROCHLOROPHYLL A METHYL PYROCHLOROPHYLLIDE A PYROPHEOPHYTIN A + METHYL PYROPHEOPHORBIDE A DERIVED FROM CHLOROPHYLL BY DECARBOMETHOXYLATION [J].

PENNINGTON, FC ;

STRAIN, HH ;

KATZ, JJ ;

SVEC, WA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1964, 86 (07) :1418-&

[7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC STUDY OF THE CHLOROPHYLL ALLOMERIZATION REACTION [J].

SCHABER, PM ;

HUNT, JE ;

FRIES, R ;

KATZ, JJ .

JOURNAL OF CHROMATOGRAPHY, 1984, 316 (DEC) :25-41

[8] REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND STRUCTURAL ASSIGNMENTS OF THE BACTERIOCHLOROPHYLLS-C [J].

SMITH, KM ;

CRAIG, GW ;

KEHRES, LA ;

PFENNIG, N .

JOURNAL OF CHROMATOGRAPHY, 1983, 281 (DEC) :209-223

[9] BACTERIOCHLOROPHYLLS-C FROM CHLOROPSEUDOMONAS-ETHYLICUM - COMPOSITION AND NMR-STUDIES OF THE PHEOPHORBIDES AND DERIVATIVES [J].

SMITH, KM ;

BUSHELL, MJ ;

RIMMER, J ;

UNSWORTH, JF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (07) :2437-2448

[10] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF BACTERIOCHLOROPHYLL-B AND ITS DERIVATIVES AS AN AID FOR STRUCTURE-ANALYSIS [J].

STEINER, R ;

WIESCHHOFF, H ;

SCHEER, H .

JOURNAL OF CHROMATOGRAPHY, 1982, 242 (01) :127-134

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