COMPARISON OF THE NMR SOLUTION STRUCTURE WITH THE X-RAY CRYSTAL-STRUCTURE OF THE ACTIVATION DOMAIN FROM PROCARBOXYPEPTIDASE-B

被引:22
作者
BILLETER, M
VENDRELL, J
WIDER, G
AVILES, FX
COLL, M
GUASCH, A
HUBER, R
WUTHRICH, K
机构
[1] SWISS FED INST TECHNOL,INST MOLEK BIOL & BIOPHYS,CH-8093 ZURICH,SWITZERLAND
[2] UNIV AUTONOMA BARCELONA,FAC CIENCIES,DEPT BIOQUIM & BIOL MOLEC,E-08193 BARCELONA,SPAIN
[3] ETS ENGN IND,CSIC,UNITAT QUIM MACROMOLEC,E-08028 BARCELONA,SPAIN
[4] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY
关键词
PROTEIN STRUCTURE; NMR SOLUTION STRUCTURE; X-RAY CRYSTAL STRUCTURE; PROCARBOXYPEPTIDASE-B; ACTIVATION DOMAIN-B;
D O I
10.1007/BF02192796
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The NMR solution structure of the activation domain isolated from porcine procarboxypeptidase B is compared with the X-ray crystal structure of the corresponding segment in the intact proenzyme. For the region of the polypeptide chain that has a well-defined three-dimensional structure in solution, i.e.. the backbone atoms of residues 11-76 and 25 amino acid side chains in this segment that form a hydrophobic core in the activation domain, the root-mean-square distance between the two structures is 1.1 angstrom. There are no significant differences in average atom positions between the two structures, but only the NMR structure shows increased structural disorder in three outlying loops located along the same edge of the activation domain. These regions of increased structural disorder in the free domain coincide only partially with the interface to the enzyme domain in the proenzyme.
引用
收藏
页码:1 / 10
页数:10
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