EVALUATION OF PEROXYOXALATE CHEMILUMINESCENCE POSTCOLUMN DETECTION OF FLUPHENAZINE IN URINE AND BLOOD-PLASMA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：20

作者：

MANN, B ^{[1
]}

GRAYESKI, ML ^{[1
]}

机构：

[1] SETON HALL UNIV,DEPT CHEM,S ORANGE,NJ 07079

来源：

BIOMEDICAL CHROMATOGRAPHY | 1991年 / 5卷 / 01期

关键词：

D O I：

10.1002/bmc.1130050111

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxyoxalate chemiluminescence may be used for sensitive postcolumn detection of phenothiazine analytes separated by high performance liquid chromatography with appropriate optimization of measurement conditions such as solvent, pH and oxalate ester. Detectability of fluorescent analytes by chemical excitation varies greatly, but analytes with low oxidation potentials are generally more readily detected at low levels, as demonstrated for phenothiazines, an important class of fluorescent drugs. Some improvement in detection limits is observed for fluphenazine when chemiluminescence detection is compared to conventional fluorescence detection. Because of the specificity of chemical excitation, fewer interferences from fluorescent impurities in a urine matrix are observed.

引用

页码：47 / 52

页数：6

共 23 条

[1] ANALYTICAL IMPLICATIONS OF THE HALF-LIFE OF THE CHEMILUMINESCENCE SIGNAL IN THE PEROXYOXALATE DETECTION SYSTEM FOR LIQUID-CHROMATOGRAPHY [J].