ELECTROCHEMICAL TITRATION OF THE CYTOCHROME HEMES IN THE RHODOPSEUDOMONAS-VIRIDIS REACTION CENTER - CYCLIC EQUILIBRIUM TITRATIONS YIELD MIDPOINT POTENTIALS WITHOUT EVIDENCE FOR HEME COOPERATIVITY

The redox and spectral characteristics of the 4-heme cytochrome c unit of the photochemical reaction center from Rhodopseudomonas viridis were studied by a combination of protein electrochemistry and spectroscopy using an ultra thin-layer spectroelectrochemical cell. Quantitative and reversible reduction of the high-potential and the low-potential hemes was performed in cyclic titrations to record the optical difference spectra in the alpha-band region. The titration of the absorbance from the high-potential hemes can be approximated with a sum of 2 Nernst functions with E(m) = 0.113 V and E(m) = 0.175 V. The corresponding titration of the absorbance from the low-potential hemes yielded E(m) = -0.257 V and E(m) = -0.175 V (all potentials quoted vs. Ag/AgCl/3 M KCl; add 0.208 V for potentials vs. standard hydrogen electrode). The high-potential hemes equilibrate rapidly and titrate identically for oxidative and reductive titrations. Under identical conditions, the low-potential hemes exhibit a hysteresis, thus indicating much slower equilibration with the applied potential. Cyclic titrations with increasing equilibration periods, however, indicate the disappearance of the hysteresis for equilibration periods approximately twice as long as for the high-potential hemes. We take this as evidence for a slower internal equilibration, but against a cooperativity of the low-potential hemes as observed for other multi-heme cytochromes.