CLONING AND CHARACTERIZATION OF THE MVRC GENE OF ESCHERICHIA-COLI K-12 WHICH CONFERS RESISTANCE AGAINST METHYL VIOLOGEN TOXICITY

被引:59
作者
MORIMYO, M
HONGO, E
HAMAINABA, H
MACHIDA, I
机构
[1] Division of Genetics, National Institute of Radiological Sciences, Inage-ku, Chiba 263
基金
日本科学技术振兴机构;
关键词
D O I
10.1093/nar/20.12.3159
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new gene mvrC conferring resistance to methyl viologen, a powerful superoxide radical propagator, was cloned on 13.5 kilo base (kb) EcoRI DNA fragment. It gave resistance against methyl viologen to even a wild-type strain with gene dosage dependence. From the physical maps obtained by restriction enzyme digestions, it was predicted to locate at 580 kbp (1 2.3 min) on the physical map of E.coli. This was confirmed by the Southern hybridization of lambda phages covering this region with mvrC probe. The DNA sequence of mvrC gene was determined and its deduced protein encoding a 12 kd hydrophobic protein was confirmed by maxicell labeling of MvrC protein.
引用
收藏
页码:3159 / 3165
页数:7
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