DEVELOPMENT AND EVALUATION OF OLIGONUCLEOTIDE DNA PROBES FOR DETECTION AND GENOTYPING OF SHIGA-LIKE TOXIN-PRODUCING ESCHERICHIA-COLI

被引：3

作者：

SAMADPOUR, M ^{[1
]}

ONGERTH, JE ^{[1
]}

LISTON, J ^{[1
]}

机构：

[1] UNIV WASHINGTON,INST FOOD SCI & TECHNOL HF-10,SEATTLE,WA 98195

来源：

JOURNAL OF FOOD PROTECTION | 1994年 / 57卷 / 05期

关键词：

ESCHERICHIA-COLI (ESCHERICHIA-COLI) O157-H7; SLTEC; OLIGONUCLEOTIDE(S); GENOTYPING; SHIGA-LIKE TOXIN;

D O I：

10.4315/0362-028X-57.5.399

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Use of antisense oligonucleotide probes (OP) for detection and genotyping of Shiga-like toxin producing Escherichia coli (SLTEC) was evaluated. Based on published deoxyribonucleic acid (DNA) sequences of the A subunits of Shiga-like toxin (SLT) I and II genes, three synthetic antisense OP were constructed (OP-1, -2 and -3). Their use for detection and genotyping of SLTEC was evaluated and the results were compared to those obtained using cloned toxiN-gene probe fragments. Both the OP-1 and OP-2 hybridized with all 69 SLT-1 and II producing strains. Furthermore, the OP-1 hybridized only with all 18 SLT-I-only producing strains, and the OP-2 hybridized only with all 48 SLT-II-only producing strains. OP-3, a pool of four OP, detected all SLTEC strains regardLess of toxin genotype. None of the OP hybridized to any of the 91 SLT-negative strains. These results demonstrate the three OP to be unique reagents for SLTEC epidemiological studies.

引用

页码：399 / 402

页数：4

共 30 条

[1] RAPID METHOD TO DETECT SHIGA TOXIN AND SHIGA-LIKE TOXIN-I BASED ON BINDING TO GLOBOTRIOSYL CERAMIDE (GB3), THEIR NATURAL RECEPTOR [J].

ASHKENAZI, S ;

CLEARY, TG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (06) :1145-1150

[2] SENSITIVE RECEPTOR-SPECIFIED ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR ESCHERICHIA-COLI VEROCYTOTOXIN [J].

BASTA, M ;

KARMALI, M ;

LINGWOOD, C .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (07) :1617-1622

[3]

BESSER R, 1992, 32 INT C ANT AG CHEM, P127

[4] HYBRIDIZATION OF ESCHERICHIA-COLI PRODUCING SHIGA-LIKE TOXIN-I, SHIGA-LIKE TOXIN-II, AND A VARIANT OF SHIGA-LIKE TOXIN-II WITH SYNTHETIC OLIGONUCLEOTIDE PROBES [J].

BROWN, JE ;

SETHABUTR, O ;

JACKSON, MP ;

LOLEKHA, S ;

ECHEVERRIA, P .

INFECTION AND IMMUNITY, 1989, 57 (09) :2811-2814

[5] NUCLEOTIDE-SEQUENCE OF THE SHIGA-LIKE TOXIN GENES OF ESCHERICHIA-COLI [J].

CALDERWOOD, SB ;

AUCLAIR, F ;

DONOHUEROLFE, A ;

KEUSCH, GT ;

MEKALANOS, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) :4364-4368

[6] ISOLATION OF ESCHERICHIA-COLI O157-H7 FROM RETAIL FRESH MEATS AND POULTRY [J].

DOYLE, MP ;

SCHOENI, JL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (10) :2394-2396

[7] H7 ANTISERUM-SORBITOL FERMENTATION MEDIUM - A SINGLE TUBE SCREENING MEDIUM FOR DETECTING ESCHERICHIA-COLI O157-H7 ASSOCIATED WITH HEMORRHAGIC COLITIS [J].

FARMER, JJ ;

DAVIS, BR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (04) :620-625

[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[9] NUCLEOTIDE-SEQUENCE ANALYSIS AND COMPARISON OF THE STRUCTURAL GENES FOR SHIGA-LIKE TOXIN-I AND SHIGA-LIKE TOXIN-II ENCODED BY BACTERIOPHAGES FROM ESCHERICHIA-COLI 933 [J].

JACKSON, MP ;

NEILL, RJ ;

OBRIEN, AD ;

HOLMES, RK ;

NEWLAND, JW .

FEMS MICROBIOLOGY LETTERS, 1987, 44 (01) :109-114

[10] EVALUATION OF OLIGONUCLEOTIDE PROBES FOR IDENTIFICATION OF SHIGA-LIKE-TOXIN-PRODUCING ESCHERICHIA-COLI [J].

KARCH, H ;

MEYER, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (06) :1180-1186

← 1 2 3 →