A SENSITIVE SANDWICH ENZYME-IMMUNOASSAY FOR MEASUREMENT OF INSULIN ON MICROTITER PLATES

A sensitive enzyme immunoassay for the measurement of insulin in human sera on microtiter plates was established. The assay is based on the sandwich technique with guinea pig anti-insulin IgG adsorbed at microtiter plate wells, human insulin as standard and the same anti-insulin IgG labeled with horseradish peroxidase. Standards used cover a range from 0 to 1200pmol/l with a detection limit of 10 pmol/1. Coefficients of variation between 3—7% for intraassay precision and 5—11% for interassay precision were obtained over the concentration range of 80—1000 pmol/1. The correlation of EIA-data with those of a commercially available double antibody radioimmuno-assay (r = 0.98) could be expressed by the equation : EIA = 0.97 RIA — 57 pmol/1. Normal fasting serum insulin concentrations in healthy subjects ranged from 11—165 pmol/1. In subjects with potentially diminished basal values concentrations of 10—79 pmol/1 were determined. The insulin response in oral glucose tolerance tests of children was discussed, who had a constitutional tall stature or Turner's syndrom, respectively. © 1990, J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart, New York. All rights reserved.