MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA AND GENOMIC DNA FOR THE HUMAN CONE TRANSDUCIN ALPHA-SUBUNIT

被引：15

作者：

KUBO, M ^{[1
]}

HIRANO, T ^{[1
]}

KAKINUMA, M ^{[1
]}

机构：

[1] HOKKAIDO UNIV,DEPT INTERNAL MED 3,SAPPORO,HOKKAIDO 060,JAPAN

来源：

FEBS LETTERS | 1991年 / 291卷 / 02期

关键词：

GTP BINDING PROTEIN; POLYMERASE CHAIN REACTION; GENOMIC ORGANIZATION; HUMAN T-CELL LEUKEMIA CELL LINE;

D O I：

10.1016/0014-5793(91)81294-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A novel GTP binding protein (G protein) alpha-subunit cDNA was isolated from a T cell leukemia cell line, Jurkat, utilizing polymerase chain reaction (PCR). The predicted amino acid sequence of this G protein alpha-subunit showed the highest identity (96.6%) to bovine cone cell-specific transducin (T(c)alpha). The organization of the coding region of this G protein alpha-subunit gene was composed of 8 exons and 7 introns. Northern hybridization revealed the presence of this G protein message in a retinoblastoma cell line, Y79. In Jurkat, however, the message was detectable only by reverse transcription/PCR. Taken together, this novel G protein alpha-subunit must be human T(c).

引用

页码：245 / 248

页数：4

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