STIMULATION OF PHOSPHOLIPASE-C ACTIVITY BY INSULIN IS MEDIATED BY BOTH ISOTYPES OF THE HUMAN INSULIN-RECEPTOR

被引：15

作者：

KELLERER, M

MACHICAO, F

SEFFER, E

MUSHACK, J

ULLRICH, A

HARING, HU ^{[1
]}

机构：

[1] INST DIABET FORSCH MUNCHEN,KOLNER PL 1,W-8000 MUNICH 40,GERMANY

[2] HORMON CHEM FORSCHUNGSLAB,MUNICH 45,GERMANY

[3] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91227-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The human insulin receptor exists in two isoforms, HIR-A and HIR-B. We studied whether both insulin receptor isotypes are able to mediate an insulin signal to phospholipase C. Plasma membranes were prepared from rat-1 fibroblasts transfected either with HIR-A or HIR-B and insulin stimulated PIP-hydrolysis was determined. We found that insulin stimulates PIP-hydrolysis in a similar dose dependent manner and to a similar extent in plasma membranes expressing HIR-A and HIR-B. These data suggest that both receptor isoforms are equally able to activate phospholipase-C. © 1991.

引用

页码：566 / 572

页数：7

共 25 条

[1] ACTIVATION OF PHOSPHATIDYLINOSITOL-3-KINASE BY INSULIN IS MEDIATED BY BOTH A-HUMAN AND B-HUMAN INSULIN-RECEPTOR TYPES [J].