SULFHYDRYL REAGENT IODOACETAMIDE INHIBITS PROGRESSION OF MEIOSIS AND SPERM TRANSFORMATION IN MOUSE OOCYTES FERTILIZED IN-VITRO

被引：1

作者：

MALESZEWSKI, M ^{[1
]}

机构：

[1] UNIV WARSAW,INST ZOOL,DEPT EMBRYOL,WARSAW,POLAND

来源：

ZYGOTE | 1995年 / 3卷 / 01期

关键词：

DISULFIDE BONDS; FERTILIZATION IN VITRO; IODOACETAMIDE; OOCYTE ACTIVATION; SPERM TRANSFORMATION;

D O I：

10.1017/S0967199400002409

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effect of iodoacetamide (LA), a sulphydryl blocking agent, on fertilisation in the mouse was examined by transferring zona-free oocytes into IA (500 mu M)-containing medium at various times after insemination. When inseminated oocytes were transferred into IA medium 10 min after insemination, the oocyte chromosomes remained aggregated in one or two masses and the sperm nucleus failed to decondense. When oocytes were transferred during the second meiosis, oocyte meiosis was more or less arrested. The sperm nucleus decondensed but remained blocked at an early stage of decondensation. These observations suggest that thiol groups in the oocyte's cytoplasm and perhaps microtubules of the meiotic spindle play crucial roles in the completion of meiosis and the transformation of sperm nucleus into pronucleus.

引用

页码：75 / 79

页数：5

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