DOLICHOL KINASE-ACTIVITY - A KEY FACTOR IN THE CONTROL OF N-GLYCOSYLATION IN INNER MITOCHONDRIAL-MEMBRANES

被引:5
作者
ARDAIL, D [1 ]
LERME, F [1 ]
LOUISOT, P [1 ]
机构
[1] UNIV LYON,LYON SUD MED SCH,DEPT BIOCHEM,CNRS,INSERM,U189,BP 12,F-69921 OULLINS,FRANCE
关键词
Dolichol kinase; Inner mitochondrial membrane; N-Glycosylation;
D O I
10.1016/0005-2736(90)90216-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inner mitochondrial membranes from liver contain a dolichol kinase which required CTP as a phosphoryl donor. Kinase activity was linear with protein concentration and unlike other reported kinases, activated almost equally well by Mg2+, Mn2+ or Ca2+. Thin-layer chromatography showed that the reaction product co-migrated with authentic dolichyl monophosphate. The phosphorylation of dolichol did not occur in presence of ATP, GTP or UTP but required exogenous dolichol for maximal activity. Newly synthesized [3H]dolichyl monophosphate has been shown to be glycosylated in the presence of GDP[14C]mannose or UDP[14C]glucose. The double labeled lipids formed by the sugar nucleotide-dependent reactions were identified respectively as [14C]mannosylphosphoryl[3H]dolichol and [14C]glucosylphosphoryl[3H]dolichol. These results are discussed in terms of regulation of N-glycosylation processes in inner mitochondrial membranes from liver. © 1990.
引用
收藏
页码:131 / 138
页数:8
相关论文
共 46 条
[1]   TOPOGRAPHY OF DOLICHYL PHOSPHATE SYNTHESIS IN RAT-LIVER MICROSOMES - TRANSBILAYER ARRANGEMENT OF DOLICHOL KINASE AND LONG-CHAIN PRENYLTRANSFERASE [J].
ADAIR, WL ;
CAFMEYER, N .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 751 (01) :21-26
[2]   CTP-DEPENDENT DOLICHOL PHOSPHORYLATION BY MAMMALIAN-CELL HOMOGENATES [J].
ALLEN, CM ;
KALIN, JR ;
SACK, J ;
VERIZZO, D .
BIOCHEMISTRY, 1978, 17 (23) :5020-5026
[3]   DOLICHOL(C55) MONOPHOSPHATASES AND PYROPHOSPHATASES OF THE RAT-LIVER [J].
APPELKVIST, EL ;
CHOJNACKI, T ;
DALLNER, G .
BIOSCIENCE REPORTS, 1981, 1 (08) :619-626
[4]   TISSUE FRACTIONATION STUDIES .5. ASSOCIATION OF ACID PHOSPHATASE WITH A SPECIAL CLASS OF CYTOPLASMIC GRANULES IN RAT LIVER [J].
APPELMANS, F ;
WATTIAUX, R ;
DUVE, CD .
BIOCHEMICAL JOURNAL, 1955, 59 (03) :438-445
[5]   GLUCOSYLTRANSFERASE ACTIVITY IN MITOCHONDRIA - BIOSYNTHESIS OF GLUCOSYL-PHOSPHORYL-DOLICHOL IN INNER MITOCHONDRIAL-MEMBRANES [J].
ARDAIL, D ;
LERME, F ;
GATEAUROESCH, O ;
LOUISOT, P ;
MORELIS, R .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1989, 21 (05) :541-548
[6]   GLYCOSYLTRANSFERASE ACTIVITIES IN LIVER-MITOCHONDRIA - PHOSPHOLIPID-DEPENDENCE OF INNER MEMBRANE MANNOSYLTRANSFERASE [J].
ARDAIL, D ;
GATEAU, O ;
MORELIS, R ;
LOUISOT, P .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 149 (03) :497-502
[7]  
ARDAIL D, 1990, IN PRESS EUR J BIOCH
[8]  
BURTON WA, 1979, J BIOL CHEM, V254, P7129
[9]  
BURTON WA, 1981, J BIOL CHEM, V256, P632
[10]   HIGH-YIELD PREPARATIVE METHOD FOR ISOLATION OF RAT-LIVER MITOCHONDRIA [J].
BUSTAMANTE, E ;
SOPER, JW ;
PEDERSEN, PL .
ANALYTICAL BIOCHEMISTRY, 1977, 80 (02) :401-408