MOLECULAR-CLONING OF HUMAN EPIDERMAL TRANSGLUTAMINASE CDNA FROM KERATINOCYTES IN CULTURE

被引：35

作者：

YAMANISHI, K ^{[1
]}

LIEW, FM ^{[1
]}

KONISHI, K ^{[1
]}

YASUNO, H ^{[1
]}

DOI, H ^{[1
]}

HIRANO, J ^{[1
]}

FUKUSHIMA, S ^{[1
]}

机构：

[1] ADV SKIN RES INST,KOHOKU KU,YOKOHAMA 223,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91651-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA encoding human epidermal transglutaminase, a key enzyme of terminal differentiation of keratinocytes. A cDNA library from cultured human keratinocytes was screened by a PCR-amplified partial cDNA fragment of the enzyme with oligonucleotide primers based on the homology of the transglutaminase family. The cDNA is 2734 bp coding a protein of 817 amino acids. The several regions including the active site cysteine residue are highly conserved among the transglutaminase family. However, the charged N-terminal domain is unique to the epidermal transglutaminase, suggesting that the region is involved in the function of the enzyme in keratinocytes. © 1991.

引用

页码：906 / 913

页数：8

共 27 条

[1] CALCIUM-REGULATED DIFFERENTIATION OF NORMAL HUMAN EPIDERMAL-KERATINOCYTES IN CHEMICALLY DEFINED CLONAL CULTURE AND SERUM-FREE SERIAL CULTURE [J].