DETERMINATION OF AMINES IN WINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL COULOMETRIC DETECTION AFTER PRECOLUMN DERIVATIZATION

被引：17

作者：

ACHILLI, G ^{[1
]}

CELLERINO, GP ^{[1
]}

DERIL, GM ^{[1
]}

机构：

[1] IRCCS,FDN IST C MONDINO,SERV ANAL,I-27100 PAVIA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 661卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)85190-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and simple method for the simultaneous evaluation of histamine, tryptamine, tyramine, phenylethylamine, putrescine, cadaverine, 1,6-diaminohexane and tryptophan in wine was developed using HPLC and coulometric detection without clean-up pretreatment. After precolumn derivatization with o-phthaldialdehyde the compounds were separated on a reversed-phase column with gradient elution. To increase the selectivity of the method a coulometric array of sixteen electrodes at increasing potentials was used. The total analysis time was less than 35 min. The analytical performance of the methods is reported. The method was used to examine the levels of various amines in three different wines.

引用

页码：201 / 205

页数：5

共 8 条

[1] IDENTIFICATION AND DETERMINATION OF PHENOLIC CONSTITUENTS IN NATURAL BEVERAGES AND PLANT-EXTRACTS BY MEANS OF A COULOMETRIC ELECTRODE ARRAY SYSTEM [J].