BIOLOGICAL-ACTIVITY AND INTRACELLULAR LOCATION OF THE TAT PROTEIN OF EQUINE INFECTIOUS-ANEMIA VIRUS

被引：7

作者：

ROSINARBESFELD, R

MASHIAH, P

WILLBOLD, D

ROSCH, P

TRONICK, SR

YANIV, A

GAZIT, A

机构：

[1] TEL AVIV UNIV,SACKLER SCH MED,DEPT HUMAN MICROBIOL,IL-69978 TEL AVIV,ISRAEL

[2] UNIV BAYREUTH,LEHRSTUHL STRUKTUR & CHEM BIOPOLYMERE,D-95440 BAYREUTH,GERMANY

[3] NCI,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892

来源：

GENE | 1994年 / 150卷 / 02期

关键词：

RECOMBINANT PROTEIN; EIAV; LENTIVIRUSES; TRANSACTIVATION; HIS TAG; PET PROKARYOTIC EXPRESSION VECTOR;

D O I：

10.1016/0378-1119(94)90443-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Tat protein of equine infectious anemia virus (EIAV) was synthesized in Escherichia coli using the inducible expression plasmid, pET16b, which contains a His.Tag leader, thus allowing for rapid and efficient enrichment of the histidine-tagged protein by metal affinity chromatography. Yields of up to 20 mg of Tat were obtained from 10(11) bacterial cells. The recombinant Tat protein was shown to potently trans-activate the EIAV long terminal repeat (LTR) following its introduction into canine cells by 'scrape loading'. The EIAV Tat protein was found to localize predominantly within the cytoplasm, in contrast to HIV-1 Tat. The availability of large amounts of purified functional EIAV Tat protein should greatly facilitate detailed structure-function analyses.

引用

页码：307 / 311

页数：5

共 32 条

[1] TRANSLATION OF EQUINE INFECTIOUS-ANEMIA VIRUS BICISTRONIC TAT-REV MESSENGER-RNA REQUIRES LEAKY RIBOSOME SCANNING OF THE TAT CTG INITIATION CODON [J].