ENHANCEMENT OF RECOMBINANT CHOLERA-TOXIN B-SUBUNIT PRODUCTION IN ESCHERICHIA-COLI BY APPLYING A FED-BATCH CONTROL STRATEGY

被引：6

作者：

MENDOZAVEGA, O

BURI, E

SPECK, D

机构：

[1] Microbial and Cell Culture Department, Transgene S.A., Strasbourg, 67082

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 10期

关键词：

D O I：

10.1007/BF00143096

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

High production levels of recombinant cholera toxin B subunit in Escherichia coli were obtained with the design of an efficient fed-batch process and control strategy. The fed-batch results demonstrated a biomass production of 58 g/L (Cell Dry Weight) attaining production levels of heterologous protein of 4.7 g/L in the intracellular fraction, 0.96 g/L exported into the periplasm and 0.27 g/L secreted into the culture supernatant.

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页码：1037 / 1042

页数：6

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