MANIPULATION OF A SINGLE-CELL WITH MICROCAPILLARY TUBING BASED ON ITS ELECTROPHORETIC MOBILITY

被引：10

作者：

KITAGAWA, S

KAWAURA, C

HASHIMOTO, O

TAKAHASHI, T

NAOI, M

TSUDA, T

机构：

[1] NAGOYA INST TECHNOL,SHOWA KU,NAGOYA,AICHI 466,JAPAN

[2] KONAN WOMANS JR COLL,KONAN,JAPAN

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 08期

关键词：

SINGLE CELL; ELECTROSTATIC FIELD; ELECTROPHORETIC MOBILITY; CAPILLARY TUBING;

D O I：

10.1002/elps.11501601225

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Manipulation of a single cell of spherical shape, approximately 5-10 mu m in diameter, was performed with capillary tubing and an electrostatic field. A single cell migrates with its electrophoretic mobility into capillary tubing against the flow of electroosmosis coming out of the capillary. After trapping the cell in the capillary, it is pulled out into the other microreservoir with the application of a reverse electric voltage. When we apply a negative voltage to the microreservoir itself, the cell in it can keep floating for a relatively long period due to electrostatic repulsion. The electrophoretic mobility of a single cell is also estimated.

引用

页码：1364 / 1368

页数：5

共 13 条

[1] OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS [J].