OCCURRENCE AND DISTRIBUTION OF PUTATIVE NEUROTRANSMITTERS IN THE FROG-LUNG PARASITE HAPLOMETRA-CYLINDRACEA (TREMATODA, DIGENEA)

被引:40
作者
MCKAY, DM
HALTON, DW
JOHNSTON, CF
FAIRWEATHER, I
SHAW, C
机构
[1] QUEENS UNIV BELFAST,SCH BIOL & BIOCHEM,DIV CELL & EXPTL BIOL,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
[2] QUEENS UNIV BELFAST,DEPT MED,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
来源
PARASITOLOGY RESEARCH | 1990年 / 76卷 / 06期
关键词
D O I
10.1007/BF00931056
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The localistion and distribution of the cholinergic, serotoninergic and peptidergic components of the nervous system of the frog-lung fluke Haplometra cylindracea have been determined by the application of standard enzyme cytochemical and immunocytochemical techniques to cryostat sections and whole-mount preparations. Cholinesterase activity (ChE), as indicative of acetylcholine, has been demonstrated cytochemically in the CNS and PNS; however, the anterior ganglia were notably unreactive. The occurrence of serotonin was examined by an indirect immunofluorescence technique, and immunoreactivity (IR) was demonstrable in small, paired anterior ganglia and in fine nerve fibres associated with the somatic muscle, cirrus and gonopore. The peptidergic protion of the nervous system was investigated using antisera to 17 mammalian regulatory peptides and the invertebrate peptide FMRFamide, and was visualised by both indirect immunofluorescence and confocal scanning laser microscopy. Positive immunostaining occurred with antisera raised against pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), substance P (SP), peptide histidine isoleucine (PHI) and FMRFamide. Immunoreactivity to PP, PYY and FMRFamide was widespread throughout the nervous system and was evident in large, paired anterior ganglia, the dorsal commissure, main nerve tracts and the extensive array of small fibres that constitute the PNS. In contrast, the distribution of nerves immunoreactive to SP and PHI was less apparent, with PHI-IR occurring exclusively within the fibrous neuropile of the ganglia and in fibres of the ventral nerve cord. Results are discussed with respect to the distribution of the various neurochemical elements and their roles as putative neurotransmitters and/or regulatory molecules. © 1990 Springer-Verlag.
引用
收藏
页码:509 / 517
页数:9
相关论文
共 38 条
[1]   BIOACTIVITY OF FMRFAMIDE AND RELATED PEPTIDES ON A CONTRACTILE SYSTEM OF THE COELENTERATE RENILLA-KOLLIKERI [J].
ANCTIL, M .
JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY, 1987, 157 (01) :31-38
[2]  
Bancroft J. D., 1967, INTRO HISTOCHEMICAL
[3]  
BARKER LR, 1966, BRIT J PHARMACOL, V52, P656
[4]   IMMUNOCYTOCHEMICAL LOCALIZATION OF REGULATORY PEPTIDES IN 6 SPECIES OF TREMATODE PARASITES [J].
BASCH, PF ;
GUPTA, BC .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1988, 91 (02) :565-570
[5]   LOCALIZATION OF BIOGENIC AMINES IN SCHISTOSOMA-MANSONI [J].
BENNETT, J ;
BUEDING, E .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY, 1971, 39 (4A) :859-&
[6]   DISTRIBUTION OF CATECHOLAMINES IN IMMATURE FASCIOLA-HEPATICA - HISTOCHEMICAL AND BIOCHEMICAL STUDY [J].
BENNETT, JL ;
GIANUTSOS, G .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1977, 7 (03) :221-225
[7]   SOME PHYSIOLOGICAL BIOCHEMICAL AND MORPHOLOGIC EFFECTS OF TRIS (P-AMINOPHENYL) CARBONIUM SALTS (TAC) ON SCHISTOSOMA MANSONI [J].
BUEDING, E ;
SCHILLER, EL ;
BOURGEOIS, JG .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1967, 16 (04) :500-+
[8]   OCCURRENCE AND CONCENTRATIONS OF BIOGENIC-AMINES IN TREMATODES [J].
CHOU, TCT ;
BENNETT, J ;
BUEDING, E .
JOURNAL OF PARASITOLOGY, 1972, 58 (06) :1098-1102
[9]   POLYPEPTIDE YY-IMMUNOREACTIVE AND NEUROPEPTIDE Y-IMMUNOREACTIVE CELLS AND NERVES IN THE ENDOCRINE AND EXOCRINE PANCREAS OF SOME VERTEBRATES - AN ONTOGENIC AND PHYLOGENETIC STUDY [J].
ELSALHY, M ;
GRIMELIUS, L ;
EMSON, PC ;
FALKMER, S .
HISTOCHEMICAL JOURNAL, 1987, 19 (02) :111-117
[10]   IMMUNOCYTOCHEMICAL DEMONSTRATION OF 5-HYDROXYTRYPTAMINE (SEROTONIN) IN THE NERVOUS-SYSTEM OF THE LIVER FLUKE, FASCIOLA-HEPATICA (TREMATODA, DIGENEA) [J].
FAIRWEATHER, I ;
MAULE, AG ;
MITCHELL, SH ;
JOHNSTON, CF ;
HALTON, DW .
PARASITOLOGY RESEARCH, 1987, 73 (03) :255-258