DISTRIBUTION OF PROFILIN IN FIBROBLASTS CORRELATES WITH THE PRESENCE OF HIGHLY DYNAMIC ACTIN-FILAMENTS

被引:110
作者
BUSS, F [1 ]
TEMMGROVE, C [1 ]
HENNING, S [1 ]
JOCKUSCH, BM [1 ]
机构
[1] UNIV BIELEFELD,CELL BIOL UNIT,POB 8640,W-4800 BIELEFELD 1,GERMANY
来源
CELL MOTILITY AND THE CYTOSKELETON | 1992年 / 22卷 / 01期
关键词
IMMONUGOLD LOCALIZATION; LYSIS-SQUIRTING; LAMELLIPODIUM; ACTIN DYNAMICS;
D O I
10.1002/cm.970220106
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have used polyclonal and monoclonal antibodies raised against calf thymus profilin to localize the corresponding protein in translocating, spreading, and stationary rat fibroblasts. Immunofluorescence of whole cells and immunogold labeling on ventral membranes of lysis-squirted cells showed that profilin was markedly enriched in the highly dynamic lamellipodia or pseudopodial lobes. Within these regions, a significant fraction was colocalized with dynamic actin filaments organized in actin ribs, cortical filaments, or stress fiber-like bundles, and little profilin was found in membrane areas appearing free of actin. In contrast, stress fibers of stationary cells as well as actin arcs and ring-like bundles of spreading and migrating cells showed very little label. These results are discussed in context with the proposed role of profilin in regional membrane dynamics typical for fibroblasts and are compared to previous data (Hartwig et al.: J. Cell Biol. 109:1571-1579, 1989) on profilin distribution in platelets and granulocytes.
引用
收藏
页码:51 / 61
页数:11
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