LOCALIZATION OF A NEW ALPHA-ACTININ BINDING-SITE IN THE COOH-TERMINAL PART OF ACTIN SEQUENCE

The interaction of filamentous actin with α-actinin, an actin cross-linking protein, is well established. On the other hand, monomeric actin - α-actinin interaction has been a subject of controversy. In this report, we have characterized the interaction of monomeric actin, coated on plastic plates under conditions of non - polymerizatioon, with α-actinin in presence of magnesium. Using specific polyclonal anti - actin antibodies, with the whole molecule or purified peptides, we have localized two sites of interaction on actin module: one near Thr-103 and a new one in the twenty last amino acids. © 1990 Academic Press, Inc.