INCREASED MDR GENE-EXPRESSION AND DECREASED DRUG ACCUMULATION IN A HUMAN COLONIC-CANCER CELL-LINE RESISTANT TO HYDROPHOBIC DRUGS

被引：15

作者：

CHAO, CCK

MA, CM

CHENG, PW

LINCHAO, S

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT GENET,STANFORD,CA 94305

[2] CHANG GUNG MED COLL,DEPT MED,TAOYUAN 33332,TAIWAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90752-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An adriamycin-resistant human colonic cancer cell line was characterized. This clone exhibits the classical multidrug resistance (MDR) phenotype, being cross-resistant to hydrophobic drugs such as colchicine, and vinblastine. In contrast, this clone shows a normal response to DNA-damaging agents. The appearance of MDR in these cells was linked to a decreased accumulation of the drug [3H]colchicine as compared to the drug-sensitive cells. This MDR line expressed 80-100 fold increased levels of the specific 4.5-kb mdr mRNA, and a gene amplification. Our results indicate that MDR in human colonic cancer cells can result from increased expression of at least one member of the mdr gene family. © 1990.

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收藏

页码：842 / 849

页数：8

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