PRP4 FROM SCHIZOSACCHAROMYCES-POMBE, A MUTANT DEFICIENT IN PRE-MESSENGER-RNA SPLICING ISOLATED USING GENES CONTAINING ARTIFICIAL INTRONS

被引：53

作者：

ROSENBERG, GH ^{[1
]}

ALAHARI, SK ^{[1
]}

KAUFER, NF ^{[1
]}

机构：

[1] DREXEL UNIV,DEPT BIOSCI & BIOTECHNOL,PHILADELPHIA,PA 19104

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 226卷 / 1-2期

关键词：

SCHIZOSACCHAROMYCES-POMBE; FISSION YEAST; TEMPERATURE-SENSITIVE MUTANTS; SPLICING; ARTIFICIAL INTRONS;

D O I：

10.1007/BF00273617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.

引用

页码：305 / 309

页数：5

共 16 条

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