A SELECTOR OF TRANSCRIPTION INITIATION IN THE PROTOZOAN PARASITE TOXOPLASMA-GONDII

被引：101

作者：

SOLDATI, D ^{[1
]}

BOOTHROYD, JC ^{[1
]}

机构：

[1] STANFORD UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,STANFORD,CA 94305

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1995年 / 15卷 / 01期

关键词：

D O I：

10.1128/MCB.15.1.87

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent development of an efficient transfection system for the apicomplexan Toxoplasma gondii allows a comprehensive dissection of the elements involved in gene transcription in this obligate intracellular parasite. We demonstrate here that for the SAG1 gene, a stretch of six repeated sequences in the region 35 to 190 bp upstream of the first of two transcription start sites is essential for efficient and accurate transcription initiation. This repeat element shows characteristics of a selector in determining the position of the transcription start sites.

引用

页码：87 / 93

页数：7

共 30 条

[1]

BANGS JD, 1992, J BIOL CHEM, V267, P9805

[2]

BENAMAR MF, 1991, NUCLEIC ACIDS RES, V19, P5857, DOI 10.1093/nar/19.21.5857

[3] TRANSCRIPTIONAL INITIATION IS CONTROLLED BY UPSTREAM GC-BOX INTERACTIONS IN A TATAA-LESS PROMOTER [J].

BLAKE, MC ;

JAMBOU, RC ;

SWICK, AG ;

KAHN, JW ;

AZIZKHAN, JC .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (12) :6632-6641

[4]

BOOTHROYD JC, IN PRESS METHODS MOL

[5]

BULOW R, 1991, J IMMUNOL, V147, P3496

[6]

BURG JL, 1988, J IMMUNOL, V141, P3584

[7] TRANSCRIPTION OF THE PROCYCLIC ACIDIC REPETITIVE PROTEIN GENES OF TRYPANOSOMA-BRUCEI [J].

CLAYTON, CE ;

FUERI, JP ;

ITZHAKI, JE ;

BELLOFATTO, V ;

SHERMAN, DR ;

WISDOM, GS ;

VIJAYASARATHY, S ;

MOWATT, MR .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (06) :3036-3047

[8] CELLULAR AND GENETIC-ASPECTS OF ANTIGENIC VARIATION IN TRYPANOSOMES [J].

CROSS, GAM .

ANNUAL REVIEW OF IMMUNOLOGY, 1990, 8 :83-110

[9] TOXOPLASMA-GONDII - REDISTRIBUTION OF MONOCLONAL-ANTIBODIES ON TACHYZOITES DURING HOST-CELL INVASION [J].

DUBREMETZ, JF ;

RODRIGUEZ, C ;

FERREIRA, E .

EXPERIMENTAL PARASITOLOGY, 1985, 59 (01) :24-32

[10] SPACER DNA-SEQUENCES UPSTREAM OF THE T-A-T-A-A-A-T-A SEQUENCE ARE ESSENTIAL FOR PROMOTION OF H2A HISTONE GENE-TRANSCRIPTION INVIVO [J].

GROSSCHEDL, R ;

BIRNSTIEL, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7102-7106

← 1 2 3 →