REDISTRIBUTION OF FODRIN IN AN IN-VITRO WOUND-HEALING MODEL OF THE CORNEAL EPITHELIUM

被引:3
作者
AMINO, K [1 ]
TAKAHASHI, M [1 ]
HONDA, Y [1 ]
FUJIMOTO, T [1 ]
机构
[1] KYOTO UNIV,FAC MED,DEPT ANAT,KYOTO 606,JAPAN
关键词
FODRIN; CORNEAL EPITHELIUM; WOUND HEALING; PROTEIN KINASE C; CALPHOSTIN C; MIGRATION; CYTOSKELETON;
D O I
10.1016/S0014-4835(05)80146-0
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
We previously observed the redistribution of a membrane skeletal protein, fodrin, after wounding in the corneal epithelium in vivo. In this study, we made an in vitro wound healing model using cultured corneal epithelial cells to investigate the redistribution mechanism of fodrin in the corneal epithelial cells. The distributional change of fodrin from the plasmalemma to the cytoplasm was observed soon after wounding by indirect immunofluorescence microscopy and laser scanning confocal microscopy. A similar change was caused by treating intact cells with phorbol-12-myristate-13-acetate (PMA), but not with calcium ionophore, A23187. The redistribution occurred even in cells pretreated with 1,2-bis(O-aminophenoxy)ethane-N,N,N' ,N'-tetraacetic acid acetomethyl ester (BAPTA-AM) before wounding. The redistribution caused by wounding or by PMA was inhibited by pretreating the cells with protein kinase C inhibitors, H-7 or calphostin C. Moreover, the reagents were found to slow down the migration of corneal epithelial cells after wounding. These results suggest that the redistribution of fodrin in the wounded corneal epithelium is caused through the activation of protein kinase C and might be related to the ensuing cell migration. (C) 1995 Academic Press Limited
引用
收藏
页码:501 / 508
页数:8
相关论文
共 37 条
[1]  
BENNETT V, 1988, J BIOL CHEM, V263, P5860
[2]   ENHANCEMENT OF THE ENDOTHELIAL PRODUCTION OF PROSTACYCLIN BY SUBSTITUTED DERIVATIVES OF BAPTA-AM [J].
BOEYNAEMS, JM ;
HEILPORN, S ;
BROEDERS, F ;
BRAEKMAN, JC .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1993, 233 (01) :13-20
[3]   INTERCELLULAR PROPAGATION OF CALCIUM WAVES MEDIATED BY INOSITOL TRISPHOSPHATE [J].
BOITANO, S ;
DIRKSEN, ER ;
SANDERSON, MJ .
SCIENCE, 1992, 258 (5080) :292-295
[4]  
BRACHER RUDOLF, 1967, INVEST OPHTHALMOL, V6, P565
[6]   INHIBITION OF PROTEIN-KINASE-C BY CALPHOSTIN-C IS LIGHT-DEPENDENT [J].
BRUNS, RF ;
MILLER, FD ;
MERRIMAN, RL ;
HOWBERT, JJ ;
HEATH, WF ;
KOBAYASHI, E ;
TAKAHASHI, I ;
TAMAOKI, T ;
NAKANO, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (01) :288-293
[7]  
BUCK RC, 1979, INVEST OPHTH VIS SCI, V18, P767
[8]  
CLEMENS MJ, 1992, J CELL SCI, V103, P881
[9]   ASSOCIATION OF FODRIN WITH BRAIN MICROTUBULES [J].
FACH, BL ;
GRAHAM, SF ;
KEATES, RAB .
CANADIAN JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY, 1985, 63 (05) :372-381
[10]   SPECTRIN REDISTRIBUTES TO THE CYTOSOL AND IS PHOSPHORYLATED DURING MITOSIS IN CULTURED-CELLS [J].
FOWLER, VM ;
ADAM, EJH .
JOURNAL OF CELL BIOLOGY, 1992, 119 (06) :1559-1572