PROPERTIES OF B-CELLS AND THY-1-ANTIGEN-EXPRESSING CELLS INFILTRATING RAT RENAL-ALLOGRAFTS

We have examined (1) the frequency of B cells secreting antibodies against donor major histocompatibility complex (MHC) antigens and (2) the properties of Thy-1-antigen-expressing leukocytes in rats rejecting renal allograft. Our results show that B cells secreting antibodies are present in the inflammatory cell population at the frequency of 1: 850. Among them only 1 out of 2-150 is engaged in production of antibodies directed to the graft MHC antigens, depending on the method of assay. This suggests that despite the observed significant production of nonspecific immunoglobulin in situ, only a minority of the B-cell population is specifically committed to the graft MHC antigens. This findings is concordant with the described previously low frequencies of the T cells specifically directed toward the graft MHC antigens. The role of the "immunologically noncommitted" cells in graft rejection is unknown. We have found that a substantial part (up to 60%) of inflammatory cells including a rat kidney allograft express the Thy-1-antigen. This suggest that they might be immature (progenitor?) cells and, therefore, unable to respond to the graft antigens. Progenitor-like properties of these cells have been confirmed their ability to reconstitute lethally irradiated syngeneic rat. Finally, these immature cells are of lymphoid, not of myeloid, lineage, because they do not proliferate in the presence of GM-CSF. © 1990.