HETEROGENEITY OF CARBOXYLESTERASES IN RAT-LIVER CELLS

被引：5

作者：

GAUSTAD, R ^{[1
]}

BERG, T ^{[1
]}

YONNUM, F ^{[1
]}

机构：

[1] UNIV OSLO,INST BIOL,DIV MOLEC CELL BIOL,N-0316 OSLO 3,NORWAY

来源：

BIOCHEMICAL PHARMACOLOGY | 1992年 / 44卷 / 04期

关键词：

D O I：

10.1016/0006-2952(92)90423-G

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Rat liver cells were separated into parenchymal cells (PC), Kupffer cells (KC) and endothelial cells (EC). The distribution of carboxylesterases (EC 3.1.1.1) between these cell types was investigated by PAGE and chromatogenic substrate staining, and compared with the results for total liver preparation and individual isoenzymes isolated by chromatofocusing. All of the fiver carboxylesterase isoenzymes could be detected in the PC, whereas in both KC and EC only those with isoelectric point (pI) 6.4/6.2 could be detected. Use of carboxylesterase inhibitors like bis-(4-nitrophenyl)phosphate and paraoxon, and organophosphorus compound hydrolase inhibitors like 4-hydroxymercuribenzoate and EDTA confirmed that these esterases were of the carboxylesterase type.

引用

页码：827 / 829

页数：3

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